Featured Resource: Primer-BLAST—NCBI’s Primer Designer and Specificity Checker
نویسنده
چکیده
This service integrates primer designing code from the popular Primer3 1 software with a specificity check that uses a custom BLAST search. Primer-BLAST eliminates the need to design primers at another site and then perform a search with those primers using the main NCBI BLAST service to check specificity. Moreover, Primer-BLAST can design primers that amplify only a particular splice variant of a gene – an important feature for PCR protocols measuring tissue specific expression. Primer-BLAST has a number of additional enhancements that make it better at picking specific primers than Primer3 and NCBI BLAST used separately.
منابع مشابه
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Primer designing for cold induced gene, DREB1A is done using Primer3 software. Seventeen sequences related to DREB1A gene were retrieved from UniGene, GenBank and RefSeq databases using Entrez. On multiple sequence alignment, a 286 bp conserved region was identified. Potential primers for the conserved region and for seventeen nucleotide sequences were determined using Primer3. The properties o...
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